The term "probiotic" comes from Greek words that indicate "for life," and its definition has changed over time. Probiotics are beneficial bacteria that, when given in sufficient amounts, provide health benefits to the host. A significant amount of probiotics should be alive and functionally active at the site of action as well as in the product for probiotics to have positive effects. The viability and sufficient amount of probiotics in the target gut determine the health benefits of probiotics. Maintaining the survival of probiotics has long been a challenge in developing viable probiotic delivery systems due to their vulnerability to many environmental conditions such as temperature and pH. Several factors, such as temperature, water activity, and other food ingredients, can reduce the viability of probiotics during manufacturing and/or storage. Because most probiotics have low thermo-resistance, high temperatures during manufacturing operations are a major cause of reduced viability.