AI in probiotic research is enabling significant breakthroughs in the understanding and application of probiotics. Through data mining and predictive analytics, AI algorithms can identify patterns in microbiome compositions that contribute to health benefits, guiding the selection of effective probiotic strains. Additionally, AI-driven technologies streamline the process of fermentation and production by predicting optimal growth conditions for probiotic microorganisms. The integration of AI in research also facilitates real-time monitoring of clinical trials, enabling faster and more accurate assessments of probiotic efficacy. With AI’s capacity to process large datasets and model complex biological systems, the future of probiotic research promises more targeted and individualized probiotic therapies, enhancing their potential in disease prevention and treatment.