Title : From theory to biotechnology and clinical practice: The world's first integrated protocol for the microbiome-gut-brain axis (1993-2002) - A forgotten pioneering system ahead of its time
Abstract:
In recent decades, the role of the microbiome in the pathogenesis and therapy of chronic diseases has received increased scientific attention, particularly in the context of the "gut-brain axis" concept. However, the systematic study of the human microbiota and its central role in maintaining health was initiated much earlier.
In 1993, the world's first "Interregional Center for Correction of Human Microbiocenosis" was opened in Novosibirsk, marking the practical implementation of a new medical paradigm. In the same year, we established the world's first industrial production of a liquid probiotic based on the Lactobacillus acidophilus 317/402 strain, creating a fundamentally new, highly active therapeutic tool.
In 1997, the monograph "Dysbiosis in the 21st Century?" first formulated and substantiated the revolutionary theory: dysbiosis is not a consequence, but a systemic cause of a wide range of chronic diseases. This work laid the foundation for a holistic medical philosophy of the future, realized at that time, which considered the role of intestinal microflora as a key link in the pathogenesis, clinical presentation, and treatment of chronic diseases.
Between 1998 and 2002, this theory was materialized into a series of patented clinical technologies, forming the world's first integrated correction protocol. The protocol included three stages:
- Environment Preparation: "Hydrogen Preconditioning." Hydrogen solutions were used for the first time worldwide for antioxidant protection and anti-inflammatory preparation of the intestinal mucosa, as well as for systemic detoxification of the recipient prior to transplantation.
- Conditioning: Use of the world's first liquid probiotic for modulating the microbial ecology.
- Radical Restoration:
- Creation of the first personalized autochthonous microbiota bank (Russian Federation patent, 1998; US patent, 2002).
- Development of the segmental microbiota transplantation method–colonoscopic introduction of microbiota in the active growth phase with topographical precision into specific sections of the large intestine.
Today, every element of this 1990s system is at the forefront of science: Liquid and metabolically active probiotics, hydrogen medicine, optimization of FMT, and the establishment of microbiota banks. This presentation will restore this forgotten system, analyze its components in light of current knowledge, and argue that it remains a remarkably prescient blueprint for medicine focused on the microbiome-gut-brain axis. The focus will be on the logic of integration—how environmental preparation, biotechnology, and microsurgical delivery methods were combined—and the lessons this offers for developing complex therapeutic ecosystems today.
Thus, the complete translational medicine cycle implemented in the 1990s–early 2000s–from revolutionary theory through biotechnological breakthrough to unique clinical methods–serves not only as proof of scientific priority but also as a relevant roadmap for genuine microbiome management in the treatment of chronic diseases.
