Title : Coculture process of Clostridium butyricum and Bacillus coagulans
Abstract:
Clostridium butyricum is a spore-forming probiotic which can promote the enhancement of benefcial bacteria and maintain intestinal microecological balance. However, it is difcult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by fask fermentation. The results showed that the optimum medium composition is 10 g/L glucose, 15 g/L corn steep powder, 15 g/L peptone, 1 g/L K2HPO4 and 0.5 g/L MnSO4.Cultured stationarily in the optimal medium for 36 h, the concentration of viable bacteria of C. butyricum DL-1 reached 1.5× 108 cfu/mL, Which was 375 times higher than that incubated in the initial medium. The sporulation rate reach 92.6%. The results revealed an economical and efective medium composition for the coculture of C. butyricum and B. coagulans, which achieved a 64.6% cost reduction. After fermentation process optimization in 10 L and 50L fermentation tank, The concentrationof viable bacteria of C. butyricum DL-1 reached 5.8× 108 cfu/mL and 9.2 cfu/mL, respectively, which is near the fermentation level of Clostridium butyricum anaerobic culture in nitrogen atmosphere. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.
Audience Take Away:
- The audience could know new probiotic strains-- Clostridium butyricum and Bacillus coagulans,
- The audience could know the properties and usage of the two strains,
- Learn a new fermentation mode, and solve the difficult they met in absolute anerobic bacteria fermentation.
