Title : In Vitro evaluation of antimicrobial activity, antibiotic susceptibility, capacity to form a biofilm isolated potential probiotic strains from Tilapia fish (Oreochromis niloticus)
Abstract:
The probiotic potential of lactic acid bacteria isolated from the gut of Tilapia fish (Oreochromis niloticus) for use in aquaculture was tested. The selection tests focused on antibacterial activity, antibiotic susceptibility and biofilm forming ability. Thus, from a collection of 154 isolates of lactic acid bacteria, only 72 isolates were able to inhibit growth of 5 pathogens Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneunomaie with inhibition diameters between 7 and 23 mm. The antibiotic sensitivity test carried out on the 72 isolates which passed the antibacterial activity test showed that only 25 lactic acid bacteria isolates were sensitive to 9 of the 12 antibiotics tested. Regarding biofilm forming ability test, all isolates (25) exhibited important capacity to form biofilm (DO≥0.5). On the hand over, others tests will be necessary before a definitive selection.
