Title : Bioactive potential of probiotics of lactic acid bacteria from bos taurus (cow) milk
Abstract:
Milk is an essential for all as it contains high valuable nutrients which are an important for all age groups. It provides basic medium for microbial production. Variety of milk and milk products basically linked with probiotic which is beneficial for intestine and boost gut flora. Different variety of milk contains various probiotic loads whether it’s raw, fermented or any other milk products. Such as yogurt & cheese formed by lactic acid bacteria (LAB), which depends upon its temperature, microbial combination, technologies involved reaction condition. Fresh or fermented milks are consumed in different regions of the world. The presence of high counts of LAB in cow milks as beneficial microbes indicates a source for explorations of biological materials of considerable public health importance and vast applications in the dairy industry (Khedid et al., 2009). It is now well understood that some of the infections and disorders such as irritable bowel syndrome, inflammatory bowel disease, & antibiotic-induced diarrhea could be treated with the use of probiotics (Shokryazdan et al., 2014). Broad range of bacteria produces peptides or bacteriocin like substance and their classification based on their molecular size, killing mechanism & chemical properties. Bacteriocins are ribosomal proteins, antimicrobial product that eradicate the growth production of other closely related microbes (Cotter et al., 2005). They are Generally Recognized as Safe (GRAS) due to less toxic as compared to unnaturally synthesize antibiotics (Holzapfel et al., 1995). The emergence of non curable infections increase death rate because of resistant to many antibiotics. The researchers are continuously searched for an alternative and the bacteriocin is an alternative to control & overcome the alarming issue of multi drug resistance.
In this study 100 milk samples were analyzed to screen the bacteriocinogenic organism. The 72 samples were meeting the criteria and were preceded for further analysis by performing the physical, chemical & microbiological standard procedures. The antimicrobial susceptibility testing used for screening potential probiotic isolates against clinical isolates.We observed 23 isolates showed activity against clinical isolates &the probiotic isolates of Lactobacillus acidophilus & Lactobacillus lactis strains showed increased bacteriocinogenic activity by producing a wide zone of inhibition against drugs resistance pathogens.
