Title : Saccharomyces Cerevisiae BT-1: A versatile probiotic with potential applications in gastrointestinal health
Abstract:
Saccharomyces cerevisiae BT-1, a unique probiotic and biotherapeutic yeast, has garnered significant attention due to its remarkable resilience to gastric acidity and antibiotics. Showing antibiotic susceptibility to antibacterial agents. Unlike many other probiotics, BT-1 does not disrupt the normal gut microbiota, making it a safe and effective option for supporting gastrointestinal health. The mechanisms underlying BT-1's efficacy in acute and chronic gastrointestinal diseases have been extensively studied. Its ability to adhere to the pathogenic(ESKAPE) organisms by trapping them. The tests of adhesion showed that, ESKAPE pathogenic bacteria tested, only Methicillin resistant staphylococcus aureus, Escherichia coli, Salmonella Typhimurium and Salmonella Typhi adhered to the surface of, Saccharomyces cerevisiae BT-1. The presence of bile salts, to some extent, inhibited this adhesion, which was not dependent on yeast viability. Electron microscopy showed that the phenomenon of yeast–bacteria adhesion occurred in vitro, coupled with its dimorphic switching to pseudohyphal differentiation under nutrient-deprived conditions, provided the synthetic low dextrose agar(SLAD) without amino acids and ammonium sulfates contributes to its prolonged survival and potential industrial applications. To further elucidate the functional characteristics of BT-1, this study investigated its total protein profile during different growth phases using SDS-PAGE. The results revealed a higher protein content in the early log phase compared to the late exponential and stationary phases, suggesting distinct metabolic activities at various stages of growth. The potential applications of BT-1 extend beyond gastrointestinal health. Its unique properties make it a promising candidate for inclusion in various dairy foods, offering an alternative probiotic option for individuals suffering from antibiotic-associated diarrhea. By understanding the mechanisms of action and functional characteristics of BT-1, Unlock potential as a valuable probiotic promoting health and well-being.
