Title : Protective effects of Lacticaseibacillus Rhamnosus GG (LGG) on dextran sulphate sodium-induced gut inflammation in zebrafish
Abstract:
Introduction:
Inflammatory bowel disease (IBD) is a kind of intestinal non-specific inflammatory disease with unclear pathogenesis which affects millions of people worldwide. The incidence of IBD is increasing year by year in many regions of the world, and the existing treatment methods have many limitations. Lactobacillus rhamnosus GG (LGG) is a well-characterized human-derived probiotic strain, which has probiotic effects such as maintaining intestinal homeostasis, regulating immunity and resisting gastrointestinal pathogens in mammals. This study was aimed to assess effects of LGG on the intestinal inflammation in the zebrafish model.
Methods:
We used dextran sulfate sodium (DSS)-treated larval zebrafish (Danio rerio, 3dpf) as an inflammatory bowel disease model, and observed the effect of LGG on DSS-induced colitis inflammation. The zebrafish labeled with neutrophils Tg (mpx:EGFP). The number and distribution of neutrophils, goblet cells and intestine mucus were observed by immuno-histochemistry, alcian blue staining and fluorescence microscopy.
Results:
The study showed that administration of LGG could reduce the expression of pro-inflammatory cytokines (TNF-α and IL-17a) induced by DSS and inhibit the recruitment of neutrophils and reduction of goblet cells to the site of intestinal inflammatory. Additionally, LGG can improve intestinal tissue damage and increase intestinal acidic mucin secretion.
Conclusion:
These findings demonstrating the potential role of LGG in the prevention and treatment of intestinal inflammation, and provide a novel therapeutic strategy to augment the existing therapeutic treatment of colitis.
