Title : Effect of synbiotics supplementation on body weight, BMI, Fasting blood glucose, HOMA-IR and microbiome profiles in individulas with obesity-induced insulin resistance
Abstract:
Background:
Obesity-induced insulin resistance (OIR) is a major public health concern associated with increased risk of type 2 diabetes, cardiovascular disease, and other metabolic disorders. Synbiotics, a combination of probiotics and prebiotics, have gained attention for their potential beneficial effects on metabolic health. This study examined the evidence for the impact of synbiotic supplementation on body weight, BMI, fasting blood glucose, HOMA-IR, and microbiota profiles in individuals with obesity-induced insulin resistance.
Methods:
This study was a randomized, double-blind placebo-controlled. Participants were allocated with randomization into 2 groups: the obese group with synbiotics supplementation (Obese+Synbiotics) and the obese group with placebo (Obese+Placebo); each group consists of 8 participants. BW, BMI, and FBG level were measured at baseline, 8 weeks after supplementation, and 4 weeks after terminating the supplementation. Changes in the microbiome profile after intervention were carried out using 16S rRNA metagenomic sequencing.
Results:
There were no significant change of body weight and BMI after 8 weeks of synbiotics supplementation and 4 weeks after supplement discontinuation, but there were significant increases in body weight by 3.38 kg and BMI by 1.37 kg/m2 in the control group. Fasting blood glucose levels were significantly decreased by 6.125 mg/dL after synbiotics supplementation. FBG did not resume 4 weeks after terminating the supplementation. In contrast, there was a significant increase of FBG in control group on week 8 and was further increased 4 weeks after placebo was discontinued. At baseline, HOMA-IR was significantly different between the two obese groups (obese+synbiotics;8.76 ± 2.937 and obese+plaebo;4.40 ± 0.738) and the non-obese group (2.13 ± 0.399). HOMA-IR in the obese+synbiotics group decreased significantly after 8 weeks of consuming synbiotics and increased slightly but not significantly after 4 weeks of stopping synbiotics. Meanwhile, in the obese+placebo group, HOMA-IR increased significantly after 8 weeks of taking placebo and continued to increase significantly after 4 weeks of stopping placebo. The number of Firmicutes increased after synbiotics intervention, while there was no change in the placebo group. The number of bacteriodetes also changed after the synbiotics intervention and there was no change in the placebo group. The Chao 1 index was higher in Obese+Synbiotics group before intervention and was further increased after synbiotics supplementation. Interestingly there were no difference for Shannon and Simpson index at baseline but increased after intervention in all groups and slightly higher in Obes+Synbiotics group.
Conclusions:
Synbiotics supplementation may prevent increase of body weight and BMI, lower fasting blood glucose levels and improves HOMA-IR in obese individuals and this may be related with improved microbiota profiles.
